![]() ![]() Northern blotting was created not long after the Southern blotting method, so the two could be used in conjunction with each other, since they both share a similar workflow. It is then sized with a complementary DNA prep. It was created as an analytical technique used in molecular biology research that allows users to measure the amount and size of very specific DNA sequences that are located in a complex combination through the immobilization of the target sequence to a solid support. Southern blotting was first developed in 1975 by Edwin Southern, hence its name. Western blotting is used to locate specific proteins in a substance, while the Northern Blot is used to determine the size and the number of RNA transcripts from a gene the lab technician is interested in. Those methods are referred to as the Northern Blot, the Southern Blot, and the Western Blot methods. All blotting techniques share a very similar workflow, which is why different methods have to be used to differentiate them from each other. The process of blotting refers to the transfer of macromolecules, including proteins and nucleic acids, from a gel that is placed onto the solid surface of an immobilized membrane so that the specific molecules can be detected. Lab technicians utilize a variety of different blots to identify the presence of whichever molecule they are focused on, including RNA, DNA, or protein, when they are testing a complex combination of related molecules. Individual Experiments Electrophoresis Packs DNA Analysis Supplies Protein Analysis SuppliesÄifferences Between a Northern Blot and a Southern Blot
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